To what extent has the toxic effect of nano-zinc oxide on human liver and kidney cells progress? Recently, I have seen people in Baidu know about this question, so today I will give you a brief introduction about this aspect.
Nano-zinc oxide has strong oxidation resistance, corrosion resistance, photocatalysis and unique UV resistance, making it widely used in optoelectronics, rubber industry, daily chemicals, food additives, biomedicine and so on. Nano-zinc oxide can enter the body through the skin, respiratory tract or digestive tract and accumulate in the body, but it is difficult for the body to eliminate such tiny substances. Therefore, potential biotoxic effects have become a hot research topic. .
In order to investigate whether nano zinc oxide has hepato-renal cytotoxicity, human normal liver cell HL-7702 and human embryonic kidney cell HEK293 were studied. Morphological observation and MTT (thiazole salt) colorimetric method were used to detect nano-oxidation. The effect of zinc on cell viability was then examined by single cell gel electrophoresis and micronucleus assays to determine the degree of cleavage of single-stranded DNA and the extent of chromosomal damage. The levels of reactive oxygen species (including GSH, MDA and SOD levels) were measured. Coomassie brilliant blue method was used to determine the carbonyl content of protein to investigate the oxidative damage of nano zinc oxide on cells and its possible mechanism. At the same time, DNA-laddering method was used to study the effect of nano zinc oxide induced apoptosis.
1. Toxic effects of nano-zinc oxide on HL-7702 and HEK293 cells The particle size distribution of nano-zinc oxide particles was determined by transmission electron microscopy. Different concentrations of zinc-containing culture medium and human normal liver cells HL-7702 and human embryonic kidney were prepared. The cells were cultured in HEK293, and their morphology was observed by an inverted microscope. The cytotoxicity was measured by MTT assay, the relative value-added rate (RGR) was calculated, and the toxicity was evaluated.
2. Genetic Toxicity of Nano-Zinc Oxide to HL-7702 and HEK293 Cells The genotoxic effects of nano-zinc oxide on human liver and kidney cells were determined by using different concentrations of nano-zinc oxide 10, 25, 50, 75 and 100 μg/mL pairs respectively. -7702 and HEK293 cells were stained for 12h, 24h and 48h, and the DNA damage of the cells was detected by single cell gel electrophoresis. The micronucleus test was used to detect the micronucleus rate of the binuclear cells after exposure, in order to obtain the in vitro genotoxicity of nano zinc oxide.
3. Study on oxidative damage of HL-7702 and HEK293 cells induced by nano-zinc oxide. Verification of whether nano-zinc oxide caused oxidative damage to HL-7702 and HEK293 cells, using different concentrations of nano-zinc oxide 10, 25, 50, 75 and 100 μg The cells were treated with /mL, and the cells were collected 24 hours later to detect the levels of glutathione GSH, malondialdehyde MDA and superoxide dismutase SOD, to explore the level of intracellular oxidative stress, and to detect the carbonyl content of the protein. Reflects the oxidative damage of proteins by nanomaterials.
A-125 zinc oxide quality index and its application
A-125 zinc oxide is one of the zinc oxide products. It belongs to inorganic chemical products.
A-125 zinc oxide molecular formula: ZnO.
A-125 zinc oxide properties: white or light yellow spherical powder, density of 5.478g / m3, specific surface area of 8 square meters / gram, high dispersion, can greatly improve the tire’s wear resistance, crack resistance, tearing Performance and tensile strength.
A-125 zinc oxide packaging: inner polyethylene film bag, jacket plastic woven bag, net weight 20 kg per bag.
A-125 Zinc Oxide Use: It is applied to internationally recognized radial tires as rubber additives to improve tire wire adhesion and tire life.